Molecular Interaction Study of Green Synthesized ZnO Nanoparticles with Lysozyme and Cytotoxicity Effect

Document Type : Original Article

Author

Department of Chemistry, Technical and Vocational University (TVU), Tehran, Iran

10.22126/bmcj.2023.9182.1002

Abstract

In this research, the biological activity of the green synthesized zinc oxide nanoparticles (ZnO NPs) through Echinophora platyloba DC. (E. platyloba) extract mediated synthesis was investigated in simulated physiological conditions (pH = 7.40). The results of the MTT assay exhibited that green synthesized ZnO NPs have efficient anticancer potential on MCF-7 cell line. Thus, it tempts us to study the synthesized ZnO NPs biocompatible aspects by interaction with biologically molecule i.e., Lysozyme (Lys). Different spectroscopic methods such as UV–visible, fluorescence, circular dichroism spectroscopic and docking simulation were employed to evaluate the structural changes in Lys in the presence of ZnO NPs. The results showed that the ZnO NPs can bind to Lys and the secondary structure of this protein remains unchanged, which is crucial in providing insights into the side effects of newly synthesized drugs on their carriers. Also, the results suggested that the interaction of ZnO NPs with Lys may be a static quenching process.

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