Biomolecule (RNA) binding analysis of [SnMe2Cl2(Me2phen)] complex: multi-spectroscopic analysis and docking simulation

Document Type : Original Article

Authors

1 Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, IRAN

2 Department of Inorganic Chemistry, Faculty of Chemistry, K.N. Toosi University of Technology, Tehran, Iran

3 Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

10.22126/bmcj.2023.2572

Abstract

In this study, the spectroscopic methods (UV–vis and fluorometric), and computational study (molecular docking) were used to investigate the interaction of [SnMe2Cl2(Me2phen)] complex with RNA under simulative physiological conditions (pH =7.40). The RNA binding properties of Sn(IV) complex exhibit that it binds to RNA through a groove binding mode and the binding constant values were computed employing the emission spectral data. The values of Ka from fluorescence measurement clearly underscore the high affinity of [SnMe2Cl2(Me2phen)] complex to RNA. The experimental results of fluorescence showed that the quenching of the complex by RNA is static. The thermodynamic parameters (∆H0 > 0 and ∆S0 > 0) are calculated by van’t Hoff equation, which demonstrated that hydrophobic interactions played major roles in the binding reaction. In this context, a negative free energy change (ΔG0 < 0) emphasizes the spontaneity of the binding process. In silico molecular docking studies further corroborated well with the experimental results.

Keywords

Files

Share

How to cite

Statistics